International Journal of Ayurveda Research

: 2010  |  Volume : 1  |  Issue : 2  |  Page : 112--121

Tinospora cordifolia (Willd.) Hook. f. and Thoms. (Guduchi) - validation of the Ayurvedic pharmacology through experimental and clinical studies

Avnish K Upadhyay1, Kaushal Kumar2, Arvind Kumar3, Hari S Mishra4,  
1 Department of Ayurved Research and Development, Patanjali Yogpeeth, Haridwar, India
2 Patanjali Herbal Garden and Agrotech Department, DYMT (SIROs), Patanjali Yogpeeth, Haridwar, India
3 Patanjali Biotech, Patanjali Ayurved Limited, Haridwar, India
4 Department of Dravya Guna, Govt. Ayurvedic College, Gurukul Kangri, Haridwar, India

Correspondence Address:
Avnish K Upadhyay
Department of Ayurved Research & Development, DYMT (SIROs), Patanjali Yogpeeth, Haridwar, Uttarakhand


T. cordifolia (Guduchi) is a large, glabrous, perennial, deciduous, climbing shrub of weak and fleshy stem found throughout India. It is a widely used plant in folk and Ayurvedic systems of medicine. The chemical constituents reported from this shrub belong to different classes, such as alkaloids, diterpenoid lactones, glycosides, steroids, sesquiterpenoid, phenolics, aliphatic compounds and polysaccharides. Various properties of T. cordifolia, described in ancient texts of Ayurveda, like Rasayana, Sangrahi, Balya, Agnideepana, Tridoshshamaka, Dahnashaka, Mehnashaka, Kasa-swasahara, Pandunashaka, Kamla-Kushta-Vataraktanashaka, Jwarhara, Krimihara, Prameha, Arshnashaka, Kricch-Hridroganashak, etc., are acquiring scientific validity through modern research adopting «DQ»reverse pharmacological«DQ» approach. Potential medicinal properties reported by scientific research include anti-diabetic, antipyretic, antispasmodic, anti-inflammatory, anti-arthritic, antioxidant, anti-allergic, anti-stress, anti-leprotic, antimalarial, hepato-protective, immuno-modulatory and anti-neoplastic activities. This review brings together various properties and medicinal uses of T. cordifolia described in Ayurveda, along with phytochemical and pharmacological reports.

How to cite this article:
Upadhyay AK, Kumar K, Kumar A, Mishra HS. Tinospora cordifolia (Willd.) Hook. f. and Thoms. (Guduchi) - validation of the Ayurvedic pharmacology through experimental and clinical studies.Int J Ayurveda Res 2010;1:112-121

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Upadhyay AK, Kumar K, Kumar A, Mishra HS. Tinospora cordifolia (Willd.) Hook. f. and Thoms. (Guduchi) - validation of the Ayurvedic pharmacology through experimental and clinical studies. Int J Ayurveda Res [serial online] 2010 [cited 2015 Feb 27 ];1:112-121
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Tinospora cordifolia (Willd.) Hook. f. and Thoms. (Guduchi) is a large, glabrous, deciduous climbing shrub belonging to the family Menispermaceae. [1],[2],[3] It is distributed throughout the tropical Indian subcontinent and China, ascending to an altitude of 300 m. In Hindi, the plant is commonly known as Giloe,[4] which is a Hindu mythological term that refers to the heavenly elixir that has saved celestial beings from old age and kept them eternally young. Other common names and synonyms are Guduchi, Amrita, Amritavalli, Madhuparni, Guduchika, Chinnobhava, Vatsadani, Tantrika, Kundalini, Chakralakshanika (Sanskrit), Gulancha (Bengali), Gurcha (Hindi), Garo, Galac (Gujarati), Thippateega (Telugu), Amrutavalli (Kannada), Amrita, Gilo (Kashmiri), Chittamrutu (Malayalam), Gulvel (Marathi), Guluchi (Oriya), Gilo (Punjabi), Seendal, Seendil Kodi (Tamil), Siddhilata, Amarlata (Assamese) Heartleaf Moonseed, Tinospora (English). [5] Guduchi, the Sanskrit name, means one which protects the entire body. The term amrita is attributed to its ability to impart youthfulness, vitality and longevity. The stems of T. cordifolia are rather succulent with long filiform fleshy aerial roots from the branches. The bark is creamy white to gray, deeply left spiraily the space in between being spotted with large rosette-like lenticels. The leaves are membranous and cordate. The flowers are small and yellow or greenish yellow. In axillary and terminal racemes or racemose panicles, the male flowers are clustered and female flowers are usually solitary. The drupes are ovoid, glossy, succulent, red and pea sized. The seeds are curved. Fruits are fleshy and single seeded. Flowers grow during summer; and fruits, during winter. [6],[7] Stem of the T. cordifolia appears in varying thicknesses, ranging from 0.6 to 5 cm in diameter; young stems are green with smooth surfaces and swelling at nodes, while the older ones show a light brown surface marked with warty protuberances due to circular lenticels; transversely smoothened surface shows a radial structure with conspicuous medullary rays traversing porous tissues; tastes bitter [Figure 1]. [5]

 Ayurvedic Pharmacology (Dravya Guna-Karma) of T. Cordifolia (Guduchi)

Ayurvedic pharmacology is based on biophysical, experiential, inferential and intuitional mechanisms. The action of a substance is based on five mechanisms of action or attributes of a substance, namely, rasa (taste appreciation of the substance by the chemical receptors on the tongue - Six tastes are described namely sweet (madhura), sour (amla), salty (lavana), bitter (tikta), pungent (kaṭu)and astringent (kaṣβya), guna (10 pairs of opposite or mirror image attributes; attribute or property of any substance), vipaka (intestinal digestion and tissue metabolism; madhura- neutral, amla- acidic, katu- alkaline), virya (potency; ushna- hot, sheeta- cold) and prabhav (specific action through specialized receptors). All these mechanisms related to drug action are biophysical in nature. Karma is the action that involves the activity or performance. It is the final effectof the drug. The properties, action (pharmacodynamics) and uses (indication) of T. cordifolia are given in [Table 1] [5],[6],[7],[8] and [Table 2]. [9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21] In the classical texts of Ayurveda, namely, Charak, Sushruta and Ashtang Sangraha and other treatises like Bhava Prakash and Dhanvantari Nighantu, etc., T. cordifolia is claimed to be useful in treating leprosy, fever, asthma, anorexia, jaundice, gout, skin infections, diabetes, chronic diarrhea, dysentery, etc. [9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21]

 Ethnobotanical, Folk and Tribal Uses of T. cordifolia (Guduchi)

There are over 400 different tribal and other ethnic groups in India. Each tribal group has its own tradition, folk language, beliefs and knowledge about use of natural resources as medicines. [22] T. cordifolia finds a special mention for its use in tribal or folk medicine in different parts of the country. Some of the important uses reported in the literature [22] are listed in the [Table 3]. Almost all the parts of the plant are documented to be useful in ethnobotanical surveys conducted by ethnobotanists. [23],[24],[25],[26]

 Phytochemical Characterization

A large number of chemicals have been isolated from T. cordifolia, belonging to different classes such as alkaloids, diterpenoid lactones, glycosides, steroids, sesquiterpenoid, phenolics, aliphatic compounds and polysaccharides. Leaves of this plant are rich in protein (11.2%), calcium and phosphorus. [27] Four new clerodane furano diterpene glucosides (amritosides A, B, C and D) have been isolated as their acetates from stems. The structures of these compounds were established on the basis of spectroscopic studies. [28] The glycosyl component of a polysaccharide from T. cordifolia has been isolated, purified, methylated, hydrolyzed, reduced and acetylated. The partially methylated alditol acetate (PMAA) derivative thus obtained have been subjected to Gas Chromatography-Mass Spectrometry (GC-MS) studies. The following types of linkages were reported: terminal-glucose, 4-xylose, 4-glucose, 4, 6-glucose and 2, 3, 4, 6-glucose. [29],[30] Callus and cell suspension cultures have been established from the stem explants of the plant. Accumulation of berberine and jatrorrhizine (protoberberine alkaloids) was observed in both callus and cell suspension cultures. [31] The signaling mechanism of the novel (1, 4)-alpha-D-glucan (RR1) isolated from T. cordifolia was investigated in macrophages to evaluate its immunostimulating properties. [32] An arabinogalactan has been isolated from the dried stems and examined by methylation analysis, partial hydrolysis and carboxyl reduction. Purified polysaccharide showed polyclonal mitogenic activity against B-cells; their proliferation did not require macrophages. [33] Detailed chemical constitution of T. cordifolia is given in [Table 4]. [34] Phytochemical characterization includes a test for one of the phytochemical components, namely, tinosporaside (limits, 0.03% to 0.04%). [35],[36]

 Reverse Pharmacological and Clinical Correlates

Indian contributions to the therapeutic revolution through reverse pharmacology will have to eventually integrate state-of-the-art high-throughput screening, combinatorial chemistry and effects of the old or novel compounds/ plants on human gene expression and proteomics. [37]

Much work has been done on T. cordifolia to validate its effects and this section describes some of these studies.

T. cordifolia has been used in Ayurvedic preparations for the treatment of various ailments throughout the centuries. It is used as a rasayana to improve the immune system and body resistance against infections. The whole plant is used medicinally; however, the stem is approved for use in medicine as listed by the Ayurvedic Pharmacopoeia of India. [5] This is due to higher alkaloid content in the stems than in the leaves. It is a traditional belief that Guduchi satva obtained from the Guduchi plant growing on neem tree (Azadirachta indica) is more bitter and more efficacious and is said to incorporate the medicinal values of neem.[38]

 Effects on Stress, Learning and Memory

T. cordifolia is known as a medhya rasayana (learning and memory enhancer) in Ayurveda. It is also described to be useful for treatment of bhrama (Vertigo) in various Ayurvedic texts. Significant response has been found in children with moderate degree of behavior disorders and mental deficit, along with improvement in IQ levels. [34]

The root of T. cordifolia is known to be used traditionally for its anti-stress activity. [22] In a 21-day randomized, double-blind placebo-controlled study, the pure aqueous extract of the root was found to enhance verbal learning and logical memory. [39] T. cordifolia has also been shown to enhance cognition (learning and memory) in normal rats and reverse cyclosporine-induced memory deficit. Both the alcoholic and aqueous extracts of T. cordifolia produced a decrease in learning scores in Hebb William maze and retention memory, indicating enhancement of learning and memory. The histopathological examination of hippocampus in cyclosporine-treated rats showed neurodegenerative changes, which were protected by T. cordifolia.[40] Various extracts of the T. cordifolia exhibited comparable anti-stress activity in mice. [41],[42]

 Anti-Inflammatory, Anti-Arthritic and Anti-Osteoporotic Activities

T. cordifolia is mentioned to treat vatarakta (gouty arthritis) and daha (burning sensation) in various Ayurvedic texts [Table 2]. It is traditionally used in compound formulations for the treatment of rheumatoid arthritis. [43] The alcoholic extract of T. cordifolia has been found to exert anti-inflammatory actions in models of acute and subacute inflammation. [44] The water extract of the stem of neem-giloe [The T. cordifolia that grow on Azadirachta indica (neem)] significantly inhibited acute inflammatory response evoked by carrageenin in a dose of 50 mg/100 g given orally and intraperitoneally. A significant inhibition of primary and secondary phases of inflammation was observed in a model of adjuvant-induced arthritis. It also significantly inhibited antibody formation by typhoid "H" antigen. A mild analgesic effect of its own as well as potentiation of morphine analgesia has been reported. [45] In another study aqueous extract of T. cordifolia showed a significat antiinflammatory effect in the cotton pellet granuloma and formalin induced arthritis model, it's effect was comparable with indomethacin and its mode of action appeared to resemble that of nonsteroidal antiinflamatory ageant. The dried stem of T. cordifolia produced significant anti-inflammatory effect in both acute and subacute models of inflammation. T. cordifolia was found to be more effective than acetylsalicylic acid in acute inflammation, although in subacute inflammation, the drug was inferior to phenylbutazone. [46] The aqueous extract of stem was reported to exert a significant anti-inflammatory effect in both cotton pellet-induced granuloma (1, 250 and 500 mg/kg given orally) and formalin-induced arthritis (1 mg/kg given orally) rat models. [47],[48]

 Anti-Allergic Activity

T. cordifolia is used for the treatment of kasa (cough) and swasa (asthma), which is described in various texts of Ayurveda [Table 2]. T. cordifolia is traditionally used for the treatment of asthma, and the juice is also employed for the treatment of chronic coughs. [49] In a clinical study, 100% relief was reported from sneezing in 83% of the patients on treatment with T. cordifolia,. Similary, there was relief from nasal discharge was reported in 69%; from nasal obstructions 61% and from nasal pruritus, in 71%. In placebo group, there was relief from sneezing only in 21% patients; from nasal discharge, in 16.2%; from nasal obstruction, in 17%; and from nasal pruritus, in 12%. Thus, T. cordifolia significantly decreased all symptoms of allergic rhinitis and was well tolerated. [50] The anti-allergic and bronchodilator properties of an aqueous extract of the stem evaluated on histamine-induced bronchospasm in guinea pigs, capillary permeability in mice and mast cell disruption in rats showed that it significantly decreased bronchospasm induced by 5% histamine aerosol, decreased capillary permeability and reduced the number of disrupted mast cells. [51],[52]

 Antioxidant Activity

T. cordifolia is mentioned as vishaghni, vishahara and tridoshashamaka in various texts of Ayurveda [Table 2]. A significant increase in the concentration of thiobarbituric acid-reactive substances (TBARS) in brain, along with its decrease in heart, was observed in diabetic rats. Tinospora cordifolia treatment decreased the concentrations of glutathione reductase (GSH) and decreased activities of superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) in the tissues of diabetics rats. Alcoholic extract of the root of T. cordifolia (TCREt) administered at a dose of 100 mg/kg orally to diabetic rats for 6 weeks normalized the antioxidant status of heart and brain. The effect of T. cordifolia root extract was better than glibenclamide (600 μ/kg) although Insulin (6 units/kg) restored all the parameters to normal status. [53],[54] T. cordifolia has also been reported to elevate GSH levels, expression of the gamma-glutamylcysteine ligase and Cu-Zn SOD genes. The herb also exhibited strong free radical-scavenging properties against reactive oxygen and nitrogen species as studied by electron paramagnetic resonance spectroscopy. [55] Aqueous extract of T. cordifolia inhibited Fenton (FeSO4) reaction and radiation - mediated 2-deoxyribose degradation in a dose-dependent fashion, with an IC50 value of 700 μ/mL for both Fenton and radiation-mediated 2-DR degradation. Similarly, it showed a moderate but dose-dependent inhibition of chemically generated superoxide anion at 500 μ/mL concentration and above, with an IC50 value of 2000 μ/mL. [56] In various studies, T. cordifolia was found effective in iron-mediated lipid damage and gamma-ray-induced protein damage, [57] amelioration of cyclophosphamide-induced toxicity, [58] alteration of lethal effects of gamma rays, [59] induction of enzymes of carcinogen/drug metabolism and inhibition of lipid peroxidation in mice, [60] free radical generation and lipid peroxidation during oxygen-glucose deprivation, [61] and nitric oxide scavenging effetcs. [62] The extract of T. cordifolia has demonstrated antioxidant action in the alloxan induced diabetes model as well. [63]

 Antineoplastic and Radio-Protective Activity

Intraperitoneal injection of the alcoholic extract of T. cordifolia has been shown to Dalton's lymphoma (DL) bearing mice e stimulated macrophage functions likephagocytosis, antigen-presenting ability and secretion of Interleukin-1 (IL-1), tumour necrosis factor (TNF) and Reference Nutrient Intake (RNI) as well as slowed tumor growth and increased lifespan of the tumor-bearing host. [64] T. cordifolia was has beem shown effective in several other tumour models including Ehrlich ascites carcinoma (EAC) in mice. [65] It induces proliferation and myeloid differentiation of bone marrow precursor cells in a tumor-bearing host, [66] activates tumor-associated macrophages-derived dendritic cells, [67] is effective against various cancers, [68] killing the cancer cells very effectively in vitro[69],[70] inhibits skin carcinogenesis in mice, [71] and inhibits experimental metastasis. [72] T. cordifolia may offer an alternative treatment strategy for cancer in combination with gamma radiation. [73],[74]

 Antipyretic and Anti-Infective Activity

Traditionally T. cordifolia is known for its jwarahara activity (antipyretic activity), as mentioned in [Table 2]. The water-soluble fraction of 95% ethanolic extract of T. cordifolia plant has shown significant antipyretic activity. [75] In another experimental study, antipyretic effects have been reported in the hexane- and chloroform-soluble portions of T. cordifolia stems. [76] Various studies show remarkable anti-infective and antipyretic properties of T. cordifolia.[77],[78] Pre-treatment with T. cordifolia was shown to impart protection against mortality induced by intra-abdominal sepsis following coecal ligation in rats and significantly reduced mortality from induced by E. coli-induced peritonitis in mice. [79]

 Hepato-Protective Activity

Various Ayurvedic preparations of T. corfifolia are indicated in pandu (anemia) and kamla (jaundice). A clinical study has shown that Guduchi plays an important role in normalization of altered liver functions (ALT, AST). [80] The antihepatotoxic activity of T. cordifolia has been demonstrated in CCl 4 induced liver damage, normallising liver function as assessed by morphological, biochemical (SGPT, SGOT, serum alkaline phosphatase, serum bilirubin) and functional (pentobarbitone sleep time) tests. T. cordifolia revealed hepatoprotective action in goats. [81] A significant increment in the functional capacities of rat peritoneal macrophages was observed following T. cordifolia treatment. [82] Addition of extract for the first 6 weeks to chloroquine showed regression of spleen by 37% to 50% after 6 weeks and 45% to 69% after 6 months from the start of treatment. Likewise, decrease in IgM and increase in Hb, as well as wellbeing (Karnofsky performance scale), were observed. [83] T. cordifolia prevents antitubercular drugs [84],[85] and bile salts [86] induced hepatic damage, x and obstructive jaundice. [87] The extract has also exhibited in vitro inactivating property against hepatitis B and E surface antigens in 48 to 72 hours. [88]

 Anti-Hyperglycemic Activity

T. cordifolia is widely used in Ayurveda for treating diabetes mellitus. [53],[58],[63] Various studies demonstrate amelioration of experimental diabetic neuropathy and gastropathy in rats, [89] reduction of blood sugar in alloxan-induced hyperglycemic rats and rabbits, [90] significant reduction in blood glucose and brain lipids, [91] increase in glucose tolerance in rodents, [92],[93] increase in glucose metabolism, [93] inhibitory effect on adrenaline-induced hyperglycemia by pyrrolidine derivative, [94],[95] and significant hypoglycemic effect in normal and alloxan diabetic rabbits [96] following administration of T. cordifolia.

 Immunomodulatory Activity

In Ayurveda T. cordifolia is believed to have rasayana (rejuvenating), balya (tonic), vayah-sthapana (anti-aging), aayushyaprada (increases the lifespan), vrishya (aphrodisiac) and chakshusya (useful in eye disorders) properties [Table 2]. The alcoholic and aqueous extracts of T. cordifolia are reported to have beneficial effects on the immune system [81],[87] and have been tested successfully for their immunomodulatory activity. [97],[98],[99],[100],[101],[102],103] The degradation of proteins due to photosensitization as assessed by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was effectively reduced by simultaneous treatment with G1-4A/PPI (partially purified immunomodulator) from T. cordifolia during photosensitization. [104] The novel (1,4)-alpha-D-glucan derived from the plant activates the immune system through the activation of macrophages via TLR6 signaling, NFkappaB translocation and cytokine production. [32],[105] Tinospora cordifolia differentially regulate elevation of cytokines as evidenced by the increased production of antiangiogenic agents IL-2 and tissue inhibitor of metalloprotease-1 (TIMP-1) in the B16F10-injected, extract-treated animals. The observed antiangiogenic activity of the plant T. cordifolia is related to the regulation of the levels of cytokines and growth factors in the blood. [106] The aqueous extract of T. cordifolia was found to enhance phagocytosis in vitro. The aqueous and ethanolic extracts also induced an increase in antibody production in vivo.[107] T. cordifolia extract (TCE) treatment caused significant reduction in eosinophil count and improved hemoglobin in HIV patients. Sixty percent patients receiving TCE and 20% on placebo reported decrease in the incidence of various symptoms associated with disease. [108] Diabetic patients with foot ulcers on T. cordifolia as an adjuvant therapy showed significantly better final outcome with improvement in wound healing. [109] Administration of T. cordifolia (200 mg/kg body weight) 1 hour before irradiation showed recovery of spleen weight from 49% of control in irradiated group to 93%; apoptosis, from 19% to 2.8%; DNA fragmentation, from 43% to 20.4%; macrophage adherence, from 75% of control to 120%; and macrophage spread size, from 8 μ to 15μ. It also stimulated proliferation in splenocytes in a dose-dependent manner. Administration of Tinospora cordifolia (Tc) before irradiation also increased levels of IL-1beta and GM-CSF from 56 pg/mL and 53 pg/mL in irradiated group to 59 pg/mL and 63 pg/mL, respectively. Similarly, radiation-induced decrease of antioxidant potential of plasma [32 Fe(2+) equiv]. as compared to control [132 Fe(2+) equiv]. was countered by administration of Tc before irradiation [74.2 Fe(2+) equiv]. RTc treatment thus suggesting its radio-protective mechanism. [110] Ten days of treatment with T. cordifolia (100 mg/kg/d) induced a significant (P vs. 38.51 ± 9.98). This suggests that activation of macrophages by T. cordifolia leads to increase in GM-CSF, which leads to leukocytosis and improved neutrophil function. [111]

 Diuretic Activity

T. cordifolia has been described as useful in mutrakriccha (urinary trouble) separately and in the form of various formulations in Ayurveda, as mentioned in [Table 2]. In a scientific study on rats and human volunteers, T. cordifolia was found to have diuretic effects. [112] It was also found effective in modulation of morphology and some gluconeogenic enzymes activity in diabetic rat kidney. [113]

 Cardioprotective Activity

Bhavprahash Nighantu and Shaligram Nighantu describe T. cordifolia to have hrudya (cardioprotective) properties and is useful in hridroga (cardiac disorders) [Table 2]. A dose-dependent reduction in infarct size and in serum and heart lipid peroxide levels were observed with prior treatment with T. cordifolia in ischemia-reperfusion-induced myocardial infarction in rats. [114] The stem extract has been normalize alterations in the lipid metabolism caused by diabetes mellitus in streptozotocin-induced diabetic rats indiectly benefiting the heart. [115] Administration of the extract of T. cordifolia roots (2.5 and 5.0 g/kg body weight) for 6 weeks resulted in a significant reduction in serum and tissue cholesterol, phospholipids and free fatty acids in alloxan diabetic rats. [116]

 Anti-Leprotic Activity

T. cordifolia is used for its kushtahara (anti-leprotic) properties, along with wide use in kandu and visarpa (types of skin disorders) and has been shown to exert anti-leprotic activity in a combination formulation. [117]

 Gastrointestinal and Anti-Ulcer Activity

Ayurvedic properties of T. cordifolia include sangrahi, arshahara, aruchinashaka, dipana, agnidipana, chardihara, trishnahara, trishnanashaka and hikkahara. Treatment with a formulation containing T. cordifolia has been shown to reduce ulcer index total acidity,with an increase in the pH of gastric fluid in pylorus-ligated rats and in the ethanol-induced gastric mucosal injury in rats. [118]

 Antifertility Activity

Oral administration of 70% methanolic extract of T. cordifolia stem to male rats at a dose level of 100 mg/d for 60 days did not cause body weight loss but decreased the weight of testes, epididymis, seminal vesicle and ventral prostate in a significant manner. [119]

 Osteoprotective Activity

Rats treated with T. cordifolia (10 mg/kg body weight) showed an osteoprotective effect, as the bone loss in tibiae was slower than that in controls. Serum osteocalcin and cross-laps levels were significantly reduced. This study demonstrates that extract of T. cordifolia has the potential for being used as antiosteoporotic agent. [120]

 Safety Pharmacology

It is a common misconception that Ayurvedic medicines are always safe. In fact, they also pose serious health risks either in the form of adverse reactions or in the form of drug interactions. In a clinical study, T. cordifolia has been shown to be at a dose of 500 mg/d for a period of 21 days in healthy individuals. [80] It has also been shown not to exert any remarkable adverse effects on the cardiovascular system, [121],[122] renal system, [112],[122] central nervous system [39].[40],[45],[121],[123] and gastrointestinal system. [49],[101],[124]


The pharmacological actions attributed to Tinospora cordifolia in Ayurvedic texts have been validated by a remarkable body of modern evidence suggesting that this drug has immense potential in modern pharmacotheraoeutics.


1Anonymous. Wealth of India: A dictionary of Indian Raw Materials and Industrial Products. 1st ed. Vol X. New Delhi: CSIR; 2003. p. 251-2.
2Aima RK. "Pictorial Guide to Plants". 1 st ed. Dehradun: Natraj Publishers. 2003. p. 454-5.
3Vaidya DB. "Materia Medica of Tibetan Medicine." Delhi: Sri Satguru Publications; 1994. p. 163.
4Bhandari C. Vanaushadhi Chandrodaya. 1 st ed., Vol. 3. Varanasi: Chaukhamba Sanskrit Sansthan; 2006. p. 86.
5The Ayurvedic Pharmacopoeia of India. Part I. 1 st ed. Vol. 1. New Delhi: Department Of AYUSH, Ministry of Health and FW; 2001. p. 53-5.
6Anonymous. Indian medicinal plants. 1 st ed. Vol. V. Kottakkal: Arya Vaidya sala; 1994. p. 283.
7Chopra RN. Chopra's Indigenous Drugs of India. Calcutta: B.K. Dhur of Academic Publishers; 1994. p. 427.
8Sharma PV. Dravyaguna Vigyan (Vegetable Drugs). 1 st ed. Vol. II. Varanasi: Chaukhambha Bharati Academy; 2003. p. 761-3.
9Chunekar KC, Pandey GS, "Guduchyadi Varga. Bhavprakash Nidhantu." Varanasi: Chaukhambha Bharati Academy; 2006. p. 269.
10Tripathi RD. Astanga Samgraha (Sutrasthana). Varanasi: Chaukhambha Sanskrit Pratisthan; 2006. p. 142,315.
11Tripathi B. Charak Samhita Part I Varanasi: Chaukhambha Surbharati Prakashan; 2003. p. 454.
12Tripathi I, "Raj Nighantu." Varanasi: Chaukhambha Krishnadas Academy; 2006. p. 31.
13Tripathi I, "Arkaprakash. Tritiya Shatak." Varanasi: Chaukhambha Krishnadas Academy; 2006. p. 45.
14Bhatta KR, Bhatta RK, Swami LR. Siddha Bhaisajya Mani Mala: Vaishwanara Hindi commentary. 3rd ed. Varanasi: Chaukhambha Krishnadas Academy; 2003. p. 31.
15Anonymous. "Shodhal Nighantu", Editor. Baroda: University Publications Sales Unit; 1978. p. 101.
16Pandit RP, "Madanpal Nighantu". Mumbai: Khemraj Srikrishnadas Prakashan; 1998. p. 8.
17Sharma PV, Sharma GP, "Kaiyadeva Nighantu". Varanasi: Chaukhambha Orientalia; 2006. p. 5.
18Kamat SD. "Dhanvantari Nighantu". Delhi: Chaukhambha Sanskrit Pratisthan; 2002. p. 1.
19Harishankar SL. Shaligram Vaishya krit Shaligram Nighantu. 3rd ed. Mumbai: Khemraj Shri Krishna Das Prakashan; 1912. p. 251-3.
20Sharma AR. Sushrut Samhita: Sushrutvimarshini Hindi Commentary along with special Deliberation etc. Part I. Varanasi: Chaukhambha Surbharati Prakashan; 2000. p. 419.
21Sharma AR. Sushrut Samhita: Sushrutvimarshini Hindi Commentary along with special Deliberation etc. Part II. Varanasi: Chaukhambha Surbharati Prakashan; 2001. p. 311,317.
22Singh J, Sinha K, Sharma A, Mishra NP, Khanuja SP. Traditional uses of Tinospora cordifolia (Guduchi). J Med Aromat Plant Sci 2003;25:748-51.
23Jain SK. Dictionary of Folk Medicine and Ethnobotany New Delhi: Deep Publishers; 1991. p. 179-80.
24Lalramnghinglova H. Ethno-Medicinal Plants of Mizoram Dehradun; Bishen Singh Mahendra Pal Singh; 2003. p. 283.
25Singh V, Pandey RP. Ethnobotany of Rajasthan India, Jodhpur: Scientific publisher (India); 1998. p. 203,231.
26Sood SK, Parmar S, Lakhanpal TN. Ethnic plants of India used in cancer cure. Dehradun: Bishen Singh Mahendra Pal Singh; 2005. p. 235.
27Khosa RL, Prasad S. Pharmacognostical studies on Guduchi (Tinospora cordifolia Miers). J Res Ind Med 1971;6:261-9.
28Maurya R, Manhas LR, Gupta P, Mishra PK, Singh G, Yadav PP. Amritosides A, B, C and D: Clerodane furano diterpene glucosides from Tinospora cordifolia. Phytochemistry 2004;65:2051-5.
29Jahfar M, Azadi P. Glycosyl composition of polysaccharide from Tinospora cordifolia. II: Glycosyl linkages. Acta Pharm 2004;54:73-8.
30Jahfar M. Glycosyl composition of polysaccharide from Tinospora cordifolia. Acta Pharm 2003;53:65-9.
31Chintalwar GJ, Gupta S, Roja G, Bapat VA. Protoberberine alkaloids from callus and cell suspension cultures of Tinospora cordifolia. Pharmaceut Biol 2003;42:81-6.
32Nair PK, Melnick SJ, Ramachandran R, Escalon E, Ramachandran C. Mechanism of macrophage activation by (1,4)-alpha-D-glucan isolated from Tinospora cordifolia. Int Immunopharmacol 2006;6:1815-24.
33Chintalwar G, Jain A, Sipahimalani A, Banerji A, Sumariwalla P, Ramakrishnan R, et al. An immunologically active arabinogalactan from Tinospora cordifolia. Phytochemistry 1999;52:1089-93.
34Singh SS, Pandey SC, Srivastava S, Gupta VS, Patro B, Ghosh AC. Chemistry and medicinal properties of Tinospora Cordifolia (Guduchi). Indian J Pharmacol 2003;35:83-91.
35Anonymous. Quality standards of Indian medicinal plants. 1st ed. Vol. I. New Delhi: ICMR; 2003. p. 212-8.
36Anonymous. Indian Herbal Pharmacopoeia Mumbai: Indian Drug Manufacturer's Association; 2002. p. 449-58.
37Vaidya A. Reverse pharmacological correlates of Ayurvedic drug actions. Indian J Pharmacol 2006;38:311-5.
38Sinha K, Mishra NP, Singh J, Khanuja SP. Tinospora cordifolia (Guduchi): A reservoir plant for therapeutic applications: A review. Indian J Trad Know 2004;3:257-70.
39Bairy KL, Rao Y, Kumar KB. Efficacy of Tinospora cordifolia on learning and memory in healthy volunteers: A double blind, randomized, placebo controlled study. Iranian J Pharmacol Therap 2004;3:57-60.
40Agarwal A, Malini S, Bairy KL, Rao MS. Effect of Tinospora Cordifolia on learning and memory in normal and memory deficit rats. Indian J Pharmacol 2002;34:339-49.
41Patil M, Patki P, Kamath HV, Patwardhan B. Anti-stress activity of Tinospora cordifolia (Wild) Miers. Indian Drugs 1997;34:211-5.
42Sen P, Maiti PC, Puri S, Ray A, Audulov NA, Valdman AV. Mechanism of anti-stress activity of Ocimum sanctum Linn, eugenol and Tinospora malabarica in experimental animals. Indian J Exp Biol 1992;30:592-6.
43The review of natural products: The most complete source of natural product information. 4th ed. In: DerMarderosian, Beutler A, John A, editors. Pennsylvania (USA): Lippincott Williams and Wilkins; 2005.
44Wesley JJ, Christina AJ, Chidambaranathan N. Effect of alcoholic extract of Tinospora Cordifolia on acute and subacute Inflammation. Pharmacologyonline 2008;3:683-7.
45Pendse VK, Dadhich AP, Mathur PN, Bal MS, Madam BR. Anti-Inflammatory, immunosuppressive and some related pharmacological actions of the water extract of Neem Giloe (Tinospora cordifolia): A Preliminary Report. Indian J Pharmacol 1977;9:221-4.
46Jana U, Chattopadhyay RN, Shw BP. Preliminary studies on anti-inflammatory activity of Zingiber officinale Rosc., Vitex negundo Linn. and Tinospora cordifolia (Willid) Miers in albino rats. Indian J Pharmacol 1999;31:232-3.
47Gulati OD, Pandey DC. Anti-inflammatory activity of Tinospora cordifolia. Rheumatism 1982;17:76-83.
48Gulati OD. Clinical trial of Tinospora cordifolia in Rheumatoid Arthritis. Rheumatism 1980;15:143-8.
49Spelman K. Traditional and clinical uses of Tinospora cordifolia, guduchi. Aust J Med Herbalism 2001;13:49-57.
50Badar VA, Thawani VR, Wakode PT, Shrivastava MP, Gharpure KJ, Hingorani LL, et al. Efficacy of Tinospora cordifolia in allergic rhinitis. J Ethnopharmacol 2005;96:445- 9.
51Nayampalli SS, Desai NK, Ainapure SS. Anti-allergic properties of Tinospora cordifolia in animal models. Indian J Pharmacol 1986;18:250-2.
52Nayampalli S, Ainapure SS, Nadkarni PM. Study of antiallergic acid Bronchodilator effects of Tinospora cordifolia. Indian J Pharmacol 1982;14:64-6.
53Prince PS, Kamalakkannan N, Menon VP. Restoration of antioxidants by ethanolic Tinospora cordifolia in alloxan-induced diabetic Wistar rats. Acta Pol Pharm 2004;61:283-7.
54Prince PS, Padmanabhan M, Menon VP. Restoration of antioxidant defence by ethanolic Tinospora cordifolia root extract in alloxan-induced diabetic liver and kidney. Phytother Res 2004;18:785-7.
55Rawal AK, Muddeshwar MG, Biswas SK. Rubia cordifolia, Fagonia cretica linn and Tinospora cordifolia exert neuroprotection by modulating the antioxidant system in rat hippocampal slices subjected to oxygen glucose deprivation. BMC Complement Altern Med 2004;13:4:11.
56Goel HC, Prem Kumar I, Rana SV. Free radical scavenging and metal chelation by Tinospora cordifolia: A possible role in radioprotection. Indian J Exp Biol 2002;40:727-34.
57Subramanian M, Chintalwar GJ, Chattopadhyay S. Antioxidant properties of a Tinospora cordifolia polysaccharide against iron-mediated lipid damage and gamma-ray induced protein damage. Redox Rep 2002;7:137-43.
58Mathew S, Kuttan G. Antioxidant activity of Tinospora cordifolia and its usefulness in the amelioration of cyclophosphamide induced toxicity. J Exp Clin Cancer Res 1997;16:407-11.
59Pahadiya S, Sharma J. Alteration of lethal effects of gamma rays in Swiss albino mice by Tinospora cordifolia. Phytother Res 2003;17:552-4.
60Singh RP, Banerjee S, Kumar PV, Raveesha KA, Rao AR. Tinospora cordifolia induces enzymes of carcinogen/drug metabolism and antioxidant system, and inhibits lipid peroxidation in mice. Phytomedicine 2006;13:74-84.
61Rawal A, Muddeshwar M, Biswas S. Effect of Rubia cordifolia, Fagonia cretica linn, and Tinospora cordifolia on free radical generation and lipid peroxidation during oxygen-glucose deprivation in rat hippocampal slices. Biochem Biophys Res Commun 2004;324:588-96.
62Jagetia GC, Baliga MS. The evaluation of nitric oxide scavenging activity of certain Indian medicinal plants in vitro: A preliminary study. J Med Food 2004;7:343-8.
63Stanely Mainzen Prince P, Menon VP. Antioxidant action of Tinospora cordifolia root extract in alloxan diabetic rats. Phytother Res 2001;15:213-8.
64Singh N, Singh SM, Shrivastava P. Immunomodulatory and antitumor actions of medicinal plant Tinospora cordifolia are mediated through activation of tumor-associated macrophages. Immunopharmacol Immunotoxicol 2005;26:145-62.
65Jagetia GC, Rao SK. Evaluation of the antineoplastic activity of guduchi (Tinospora cordifolia) in Ehrlich ascites carcinoma bearing mice. Biol Pharm Bull 2006;29:460-6.
66Singh SM, Singh N, Shrivastava P. Effect of alcoholic extract of Ayurvedic herb Tinospora cordifolia on the proliferation and myeloid differentiation of bone marrow precursor cells in a tumor-bearing host. Fitoterapia 2006;77:1-11.
67Singh N, Singh SM, Shrivastava P. Effect of Tinospora cordifolia on the antitumor activity of tumor-associated macrophages-derived dendritic cells. Immunopharmacol Immunotoxicol 2005;27:1-14.
68Mittal A, Singh RP. Anticancer and immunomodulatory properties of Tinospora, K.G.Ranawat (ed.) herbal drugs: Ethnomedicine to modern medicine.DOI 10.1007/978-3-540-79116-4_12 Berlin Heidelberg: Springer; 2009. p. 195.
69Jagetia GC, Nayak V, Vidyasagar MS. Evaluation of the antineoplastic activity of guduchi (Tinospora cordifolia) in cultured HeLa cells. Cancer Lett 1998;127:71-82.
70Jagetia GC, Rao SK. Evaluation of cytotoxic effects of dichloromethane extract of guduchi (Tinospora cordifolia Miers ex Hook F and THOMS) on cultured HeLa cells. Evid Based Complement Alternat Med 2006;3:267-72.
71Chaudhary R, Jahan S, Goyal PK. Chemopreventive potential of an Indian medicinal plant (Tinospora cordifolia) on skin carcinogenesis in mice. J Environ Pathol Toxicol Oncol 2008;27:233-43.
72Leyon PV, Kuttan G. Inhibitory effect of a polysaccharide from Tinospora cordifolia on experimental metastasis. J Ethnopharmacol 2004;90:233-7.
73Rao SK, Rao PS, Rao BN. Preliminary investigation of the radiosensitizing activity of guduchi (T. cordifolia) in tumor-bearing mice. Phytother Res 2008;22:1482-9.
74Goel HC, Prasad J, Singh S, Sagar RK, Agrawala PK, Bala M, et al. Radioprotective potential of an herbal extract of Tinospora cordifolia. J Radiat Res (Tokyo) 2004;45:61-8.
75Vedavathy S, Rao KN. Antipyretic activity of six indigenous medicinal plants of Tirumala Hilla, Andhra Pradesh, India. J Ethnopharmacol 1991;33:193-6.
76Ikram M, Khattak SG, Gilani SN. Antipyretic studies on some indigenous Pakistani medicinal plants: II. J Ethnopharmacol 1987;19:185-92.
77Jeyachandran R, Xavier TF, Anand SP. Anti-bacterial activity of stem extracts of Tinospora cordifolia (willd). Ancient Science life 2003;23:40-4.
78Gupta KC, Viswanathan R. Antituberculous substances from plants. Antibiot Chemother 1956;6:194-5.
79Thatte UM, Kulkarni MR, Dahanukar SA. Immunotherapeutic modulation of E. coli peritonitis and bacteremia by Tinospora cordifolia. J Postgrad Med 1992;38:13-5.
80Karkal YR, Bairy LK. Safety of aqueouse of Tinospora cordifolia (Tc) in healthy volunteers: A double blind randomised placebo controlled study. Iranian J Pharmacol Therap 2007;6:59-61.
81Nagarkatti DS, Rege NN, Desai NK, Dahanukar SA. Modulation of Kupffer cell activity by Tinospora cordifolia in liver damage. J Postgrad Med 1994;40:65-7.
82Bishayi B, Roychowdhury S, Ghosh S, Sengupta M. Hepatoprotective and immunomodulatory properties of Tinospora cordifolia in CCl4 intoxicated mature albino rats. J Toxicol Sci 2002;27:139-46.
83Singh RK. Tinospora cordifolia as an adjuvant drug in the treatment of hyper-reactive malarious splenomegaly: Case reports. J Vector Borne Dis 2005;42:36-8.
84Adhvaryu MR, Reddy N, Vakharia BC. Prevention of hepatotoxicity due to anti tuberculosis treatment: A novel integrative approach. World J Gastroenterol 2008;14:4753- 62.
85Panchabhai TS, Ambarkhane SV, Joshi AS, Samant BD, Rege NN. Protective effect of Tinospora cordifolia, Phyllanthus emblica and their combination against antitubercular drugs induced hepatic damage: An experimental study. Phytother Res 2008;22:646-50.
86Upadhyay L, Mehrotra A, Srivastava AK, Rai NP, Tripathi K. An experimental study of some indigenous drugs with special reference to hydraulic permeability. Indian J Exp Biol 2001;39:1308-10.
87Rege N, Bapat RD, Koti R, Desai NK, Dahanukar S. Immunotherapy with Tinospora cordifolia: A new lead in the management of obstructive jaundice. Indian J Gastroenterol 1993;12:5-8.
88Mehrotra R, Katiyar CK, Gupta AP. Hepatoprotective compositions and composition for treatment of conditions related to hepatitis B and E infection. US Patent 749296; 2000.
89Grover JK, Rathi SS, Vats V. Amelioration of experimental diabetic neuropathy and gastropathy in rats following oral administration of plant (Eugenia jambolana, Mucuna pruriens and Tinospora cordifolia) extracts. Indian J Exp Biol 2002;40:273-6.
90Raghunathan K, Sharma PV. The aqueous extract of T. cordifolia caused reduction of blood sugar in alloxan induced hyperglycemic rats and rabbits. J Res Ind Med 1969;3:203-9.
91Dhaliwal KS. Method and composition for treatment of diabetes. US Patent 5886029; 1999.
92Gupta SS, Verma SC, Garg VP, Rai M. Antidiabetic effect of Tinospora cordifolia. I: Effect on fasting blood sugar level, glucose tolerence and adrenaline induced hyperglycemia. Indian J Med Res 1967;55:733-45.
93Grover JK, Vats V, Rathi SS, Dawar R. Traditional Indian anti-diabetic plants attenuate progression of renal damage in streptozotocin induced diabetic mice. J Ethnopharmacol 2001;76:233-8.
94Grover JK, Vats V, Rathi SS. Anti-hyperglycemic effect of Eugenia jambolana and Tinospora cordifolia in experimental diabetes and their effects on key metabolic enzymes involved in carbohydrate metabolism. J Ethnopharmacol 2000;73:461-70.
95Stanely P, Prince M, Menon VP. Hypoglycaemic and other related actions of Tinospora cordifolia roots in alloxan-induced diabetic rats. J Ethnopharmacol 2000;70:9-15.
96Wadood N, Wadood A, Shah SA. Effect of Tinospora cordifolia on blood glucose and total lipid levels of normal and alloxan-diabetic rabbits. Planta Med 1992;58:131-6.
97Rege NN, Nazareth HM, Bapat RD, Dahanukar SA. Modulation of immunosuppression in obstructive jaundice by Tinospora cordifolia. Indian J Med Res 1989;90:478-83.
98Manjrekar PN, Jolly CI, Narayanan S. Comparative studies of the immunomodulatory activity of Tinospora cordifolia and Tinospora sinensis. Fitoterapia 2000;71:254-7.
99Dikshit V, Damre AS, Kulkarni KR, Gokhale A, Saraf MN. Preliminary screening of imunocin for immunomodulatory activity. Indian J Pharm Sci 2000;62:257.
100Dahanukar SA, Thatte UM, Pai N, More PB, Karandikar SM. Immunotherapeutic modification by Tinospora cordifolia of abdominal sepsis induced by caecal ligation in rats. Indian J Gastroenterol 1988;7:21-3.
101Rege NN, Thatte UM, Dahanukar SA. Adaptogenic properties of six rasayana herbs used in Ayurvedic medicine. Phytother Res 1999;13:275-91.
102Thatte UM, Dahanukar SA. Immunotherapeutic modification of experimental infections by Indian medicinal plants. Phytother Res 1989;3:43-9.
103Thatte UM, Chhabria S, Karandikar SM, Dahanukar SA. Immunotherapeutic modification of E. coli induced abdominal sepsis and mortality in mice by Indian medicinal plants. Indian Drugs 1987;25:95-7.
104Desai VR, Kamat JP, Sainis KB. An immunomodulator from Tinospora cordifolia with antioxidant activity in cell-free systems. Proc Indian Acad Sci (Chem Sci) 2002;114:713-9.
105Nair PK, Rodriguez S, Ramachandran R, Alamo A, Melnick SJ, Escalon E, et al. Immune stimulating properties of a novel polysaccharide from the medicinal plant Tinospora cordifolia. Int Immunopharmacol 2004;4:1645-59.
106Leyon PV, Kuttan G. Effect of Tinospora cordifolia on the cytokine profile of angiogenesis-induced animals. Int Immunopharmacol 2004;4:1569-75.
107Ranjith MS, Ranjitsingh AJ, Gokul Shankar S, Vijayalaksmi GS, Deepa K, Sidhu HS. Enhanced phagocytosis and antibody production by Tinospora cordifolia: A new dimension in Immunomodulation. Afr J Biotechnol 2008;7:81-5.
108Kalikar MV, Thawani VR, Varadpande UK, Sontakke SD, Singh RP, Khiyani RK. Immunomodulatory effect of Tinospora cordifolia extract in human immuno-deficiency virus positive patients. Indian J Pharmacol 2008;40:107-10.
109Purandare H, Supe A. Immunomodulatory role of Tinospora cordifolia as an adjuvant in surgical treatment of diabetic foot ulcers: A prospective randomized controlled study. Indian J Med Sci 2007;61:347-55.
110Singh L, Tyagi S, Rizvi MA, Goel HC. Effect of Tinospora cordifolia on gamma ray-induced perturbations in macrophages and splenocytes. J Radiat Res (Tokyo) 2007;48:305-15.
111Thatte UM, Rao SG, Dahanukar SA. Tinospora cordifolia induces colony stimulating activity in serum. J Postgrad Med 1994;40:202-3.
112Nayampalli SS, Ainapure SS, Samant BD, Kudtarkar RG, Desai NK, Gupta KC. A comparative study of diuretic effects of Tinospora cordifolia and hydrochloro-thiazide in rats and a preliminary phase I study in human volunteers. J Postgrad Med 1988;34:233-6.
113Nagaraja PK, Kammar KF, Devi S. Modulation of morphology and some gluconeogenic enzymes activity by Tinospora cordifolia (Willd.) in diabetic rat kidney. Biomed Res 2007;18:179-83.
114Rao PR, Kumar VK, Viswanath RK, Subbaraju GV. Cardioprotective activity of alcoholic extract of Tinospora cordifolia in ischemia-reperfusion induced myocardial infarction in rats. Biol Pharm Bull 2005;28:2319-22.
115Nagaraja PK, Kammar KF, Devi S. Efficacy of Tinospora cordifolia (Willd.) extracts on blood lipid profile in streptozotocin diabetic rats: Is it beneficial to the heart? Biomedical Research 2008;19:92-6.
116Stanely Mainzen Prince P, Menon VP, Gunasekaran G. Hypolipidaemic action of Tinospora cordifolia roots in alloxan diabetic rats. J Ethnopharmacol 1999;64:53-7.
117Asthana JG, Jain S, Mishra A, Vijaykant MS. Evaluation of antileprotic herbal drug combinations and their combination with Dapsone. Indian Drugs 2001;38:82-6.
118Bafna PA, Balaraman R. Anti-ulcer and anti-oxidant activity of pepticare: A herbomineral formulation. Phytomedicine 2005;12:264-70.
119Gupta RS, Sharma A. Antifertility effect of Tinospora cordifolia (Willd.) stem extract in male rats. Indian J Exp Biol 2003;41:885-9.
120Kapur P, Jarry H, Wuttke W, Pereira BM, Seidlova-Wuttke D. Evaluation of the antiosteoporotic potential of Tinospora cordifolia in female rats. Maturitas 2008;59:329-38.
121Dhar ML, Dhar MM, Dhawan BN, Mehrotra BN, Ray C. Screenig of Indian Plants for biological activity: Part I. Indian J Exp Biol 1968;6:232-47.
122Singh KP, Gupta AS, Pendse VK, Mahatma CP, Bhandari DS and Mahawar MM. Experimental and clinical studies on Tinospora cordifolia. J Res Indian Med 1975;10:9-14.
123Kundnani KM, Mahajan VR, Jolly CI. A new hypoglycaemic agent from Tinospora cordifolia: Miers. Indian Drugs 1985;23:119-20.
124Sheth MD, Rege NN, Dahanukar SA. Effect of Tinospora cordifolia on gastrointestinal dismotality induced by chronic, unprecdictable wrap-restraint. Indian J Pharmacol 2001;33:135.