International Journal of Ayurveda Research

ORIGINAL ARTICLE
Year
: 2010  |  Volume : 1  |  Issue : 4  |  Page : 243--246

Validated HPLC-UV method for the determination of berberine in raw herb Daruharidra (Berberis aristata DC), its extract, and in commercially marketed ayurvedic dosage forms


Anubhuti Pasrija, Rahul Singh, Chandra Kant Katiyar 
 Dabur Research and Development Centre, Dabur India Limited, Plot No.22, Site IV, Sahibabad-201 010, Ghaziabad (U.P.), India

Correspondence Address:
Rahul Singh
Dabur India Limited, Plot No.22, Site IV, Sahibabad, Ghaziabad
India

A new, simple, sensitive, selective, and precise high-performance liquid chromatography (HPLC) method for analysis of berberine in crude plant material, herbal extract, and ayurvedic dosage forms was developed and validated. The stationary phase was inert sil C 18 column . The mobile phase consisting of acetonitrile (HPLC Grade) and potassium dihydrogen phosphate buffer (pH 2.5) in a gradient flow was used.The column was equilibrated with the mobile phase (flow rate 1.0 ml/min); elution was monitored at 346 nm. The linear regression analysis data for the calibration plots showed good linear relationship, with r 2 = 0.9942 in the concentration range of 16380-30420 μg/ml with respect to the peak area. The method was validated for specificity, precision, recovery, and linearity according to the International Conference on Harmonization guidelines. Statistical analysis of the data showed that the method is reproducible and selective for the estimation of berberine.


How to cite this article:
Pasrija A, Singh R, Katiyar CK. Validated HPLC-UV method for the determination of berberine in raw herb Daruharidra (Berberis aristata DC), its extract, and in commercially marketed ayurvedic dosage forms.Int J Ayurveda Res 2010;1:243-246


How to cite this URL:
Pasrija A, Singh R, Katiyar CK. Validated HPLC-UV method for the determination of berberine in raw herb Daruharidra (Berberis aristata DC), its extract, and in commercially marketed ayurvedic dosage forms. Int J Ayurveda Res [serial online] 2010 [cited 2014 Oct 21 ];1:243-246
Available from: http://www.ijaronline.com/article.asp?issn=0974-7788;year=2010;volume=1;issue=4;spage=243;epage=246;aulast=Pasrija;type=0